CCT eta Antibody | PK/16/8/a

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CCT eta Antibody | PK/16/8/a gallery image 1

Western blot analysis of HeLa whole cell lysate (1) and HeLa heat stressed whole cell lysate (2) probed with Rat anti CCT eta (MCA2179) followed by F(ab')2 Rabbit anti Rat IgG conjugated to HRP and visualized by chemiluminescence

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Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
CCT-eta but not CCT-beta protein and mRNA are differentially expressed in fetal versus adult fibroblasts. RNA and protein extracted from fetal and adult fibroblasts were subjected to qRT PCR (a & b) and Western blot (c & d) analyses respectively. CCT-eta mRNA was significantly more abundant in adult fibroblasts when compared to fetal fibroblasts (a); there was no significant difference in CCT-beta message levels between fetal and adult fibroblasts (b). Values are means ± SEM of three independent studies performed in duplicate. Statistical analyses were performed using Student's t test. NS = non-significant. Equal amounts of protein loaded from fetal and adult fibroblasts showed that adult fibroblasts express significantly greater CCT-eta protein(c). In contrast CCT-beta protein levels were not different between fetal and adult fibroblasts (d). Blots shown here are representative of at least three different experiments.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010) Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.

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CCT eta Antibody | PK/16/8/a gallery image 3

Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
siRNAs against CCT-eta and CCT-beta decrease both basal and EGF- induced mRNA and protein levels of their targets in fibroblasts. (a & b) qRT-PCR analysis of CCT-eta and CCT-beta mRNA levels showed effective inhibition of both basal expression and EGF-induction in siRNA-transfected adult fibroblasts. Results are expressed as relative quotient (RQ) of measured CCT-eta or CCT-beta mRNA and were calculated as a percentage of baseline control levels (100%). Values are means ± SEM of six independent studies, each performed in duplicate. Statistical analyses were performed with Student's t test. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA. (c & d) Western blot results using CCT-eta and CCT-beta antibody (1:500) showed effective reduction of CCT-eta and CCT-beta protein levels when siRNA was administered but no decrease when scrambled siRNA was employed. GAPDH was used as a loading control. A representative immunoblot of up to four similar such blots is shown for each analysis.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010) Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.

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CCT eta Antibody | PK/16/8/a gallery image 4

Published customer image:
Rat anti CCTη antibody, clone PK/16/8/a used for the detection of CCT&eta in fetal and adult rabbit fibroblasts by western blotting.
Image caption:
siRNA downregulation of CCT-eta but not CCT-beta reduces a-SMA protein levels in adult fibroblasts. (a & b)Western blot showed effective reduction of both CCT-eta and a-SMA protein levels when CCT-eta siRNA was administered, leaving beta-actin largely unaffected. In contrast, downregulating CCT-beta did not lead to significant reduction in either a-SMA or beta-actin levels. GAPDH was used as loading internal control. A representative immunoblot of up to three similar such blots are shown for each analysis. Ntx- no transfection; EGF-EGF treatment (1 nM); siRNA-treatment with CCT-eta/CCT-beta siRNA; Scr- treatment with scrambled control siRNA.

From: Satish L, Johnson S, Wang JH-C, Post JC, Ehrlich GD, et al. (2010) Chaperonin Containing T-Complex Polypeptide Subunit Eta (CCT-eta) Is a Specific Regulator of Fibroblast Motility and Contractility. PLoS ONE 5(4): e10063.

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  • Rat anti CCT eta
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  • Product Type
    Monoclonal Antibody
  • Clone
    PK/16/8/a
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2179P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2179
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti CCTeta antibody, clone PK/16/8/a recognizes the eta polypeptide of the CCT chaperonin molecule complex.CCTη is a 543 amino acid ~60kDa molecule encoded by the CCT7 gene, a member of the TCP-1 chaperonin family (UniProt: Q2NKZ1)

      The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumour suppressor protein.

      Rat anti CCTeta antibody, clone PK/16/8/a detects a band of ~60kDa in HeLa cell lysates using western blotting under reducing conditions.
    • Intended Use
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Humanyes
      Bovineyes
      Rabbityes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/500
      Immunohistology - Paraffin(1)1/100
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human prostate.
    • Immunofluorescence
    • Western Blotting
      MCA2179 detects a band of approximately 60kD in Hela cell lysates.
    • Instructions For Use

    Additional CCT eta Antibody Formats

    Formats Clone Applications Sizes available
    CCT eta Antibody : Purified PK/16/8/a P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human prostate.
              • Application NameReference Images
                Western Blotting

              References

              Further Reading

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