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Cathepsin K

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Staining of staurosporine-induced THP-1 cells using the Magic Red Cathepsin K kit

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  • Magic Red™ Cathepsin K Kit
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  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ICT939IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf25 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • The Magic Red Cathepsin K kit uses a quick and easy method to analyze intracellular Cathepsin K protease activity in whole living cells.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Magic Red MR-(LR)2 Reagent - lyophilized
      Hoechst 33342 Stain, 1 mL
      Acridine orange (AO), 0.5 mL
    • Preparing The Antibody
    • Test Principle
      Cathepsin Magic Red kits measure cathepsin K protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product.

      The Magic Red reagent contains a cathepsin K target sequence peptide (LR)2 linked to a red (Cresyl Violet) fluorescent probe.

      The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits.

      This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.
    • Buffer Solution
    • Storage
      Store the unopened kit (and each unopened component) at +4oC until the expiration date.
      Protect the MR-(LR)2 reagent from light at all times.

      Once reconstituted, the 260X MR-(LR)2 stock should be stored at -20°C protected from light.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
      Magic Red is a trademark of Immunochemistry Technologies, LLC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT939.pdf

    Additional Cathepsin K Formats

    Formats Applications Sizes available
    Cathepsin K : Magic Red™ (Red Fluorescence) IF 25 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Cathepsin K thumbnail image 1


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