Caspase-10

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Caspase-10 gallery image 1

Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases

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  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ICT923F, IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf100 Tests
    ICT923
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • The FAM FLICA Caspase 10 kit uses a quick and easy method to analyze active caspases in apoptotic cells.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      4 vials of FAM-AEVD-FMK FLICA Reagent - lyophilized
      10X Wash Buffer, 60 mL
      Fixative, 6 mL
      Propidium Iodide, 1 mL
      Hoechst Stain, 1 mL
    • Preparing The Antibody
    • Test Principle
      Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

      The Caspase FLICA Kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

      This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
    • Buffer Solution
    • Storage
      Store the unopened kit (and each unopened component) at +4oC until the expiration date.
      Protect the FLICA reagent from light at all times.

      Once reconstituted, the 150X FLICA stock should be stored at -20°C protected from light.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
      FLICA is a trademark of Immunochemistry Technologies, LLC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT923.pdf

    Additional Caspase-10 Formats

    Formats Applications Sizes available
    Caspase-10 : FAM (Green Fluorescence) F, IF 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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