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Most immunoassays including ELISA, immunohistochemistry, western blot and flow cytometry rely on reagents for experimental success. These reagents include wash buffers, blocking solutions, assay diluents, and substrate buffers, all of which are crucial for accurate and consistent results. For immunohistochemistry in particular, most formalin fixed tissues require an antigen retrieval step prior to staining. Antigen retrieval buffers function to facilitate epitope retrieval to improve binding of the primary antibody. They also allow for excellent preservation of tissue morphology, which can improve staining results.
The success of ELISAs heavily depends on adequate and thorough washing between antibody incubation steps in order to remove unbound antibody, reduce background noise, and increase signal specificity. Therefore, reagents are an indispensable part of your research protocol and necessary for analyses such as measuring cell viability or removing mycoplasma from cell culture. As such, Bio-Rad offers a wide selection of reagents for various applications.
Our reagents are designed with your experiment in mind and provide excellent performance to help you achieve excellent results. We offer convenient ready to use and concentrated formats in a number of sizes, depending on your experimental needs. Our reagents are easy to use and offer reliable consistent formulation. Some of our most highly cited reagents include the Block Ace (12 papers) and ELISA Synblock buffers (15 papers) and the alamarBlue® cell proliferation reagent (22 papers).
Can’t find what you’re looking for? Bio-Rad offers custom antibody services including monoclonal and polyclonal antibody generation, antibody production and modification services. Just complete the short inquiry form and one of our specialists will contact you to discuss your research needs.
For assistance with your experiments, visit our resources section for detailed protocols, tips, guides and educational articles. For more information or assistance with choosing an appropriate buffer or accessory reagent, please contact our technical support specialists.