BrdU (bromodeoxyuridine)

Seeing double?

Seeing double?

Anti-BrdU Antibody promotion

To support your cell proliferation experiments, this June, we offer you a free vial of selected Rat Anti-BrdU Antibody, clone BU1/75, products with every vial of Mouse Anti-BrdU Antibody, clone Bu20a*. Our four BrdU antibody double deals are based on like-for-like format matches, and available for a limited time only (offer expires June 30, 2017).

Benefits of the anti-BrdU antibody double deals:
 

Our anti-BrdU antibody double deals not only enable you to detect BrdU but also other thymidine analogs such as 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU), 5′-ethynyl-2′-deoxyuridine (EdU) and the drug trifluridine when incorporated into DNA (Aten et al. 1992, Liboska et al. 2012, Kitao et al. 2016).

The different cross-reactivities of the two anti-BrdU antibodies to incorporated thymidine analogs and trifluridine are shown in Table 1.

Table 1. Cross-reactivities of the Mouse Anti-BrdU Antibody, clone Bu20a, and Rat Anti-BrdU Antibody, clone BU1/75, to the thymidine analogs BrdU, CldU, EdU, IdU, and the drug trifluridine.

 

Thymidine Analog Cross-Reactivity

Trifluridine Cross-Reactivity

Anti-BrdU Antibody clone

BrdU

CldU

EdU

IdU

Trifluridine

Mouse Anti-BrdU Antibody (clone Bu20a)

+

+

+

+

+

Rat Anti-BrdU Antibody (clone BU1/75)

+

+

+

-

-

Table adapted from Aten et al. (1992) (BrdU, CldU, and IdU). Cross-reactivity to EdU is based on Liboska et al. (2012) while detection of incorporated trifluridine has been reported by Kitao et al. (2016). Abbreviations: + = reported cross-reactivity, - = no cross-reactivity.

Double deal overview:
 

0.2 mg size anti-BrdU antibody double deal 1 – Promo code BrdU-DD1:
Buy one vial of Mouse Anti-BrdU Antibody (MCA2483, 0.2 mg) and get one vial of Rat Anti-BrdU Antibody (MCA2060, 0.2 mg) for free

Buy MCA2483

0.1 mg size anti-BrdU antibody double deal 2 – Promo code BrdU-DD2:
Buy one vial of Mouse Anti-BrdU Antibody (MCA2483GA, 0.1 mg) and get one vial of Rat Anti-BrdU Antibody (MCA2060GA, 0.1 mg) for free

Buy MCA2483GA

Test size anti-BrdU antibody double deal 3 – Promo code BrdU-DD3:
Buy one vial of Mouse Anti-BrdU Antibody (MCA2483T, 20 µg test size) and get one vial of Rat Anti-BrdU Antibody (MCA2060T, 20 µg test size) for free

Buy MCA2483T

Green fluorophore conjugated anti-BrdU antibody double deal 4 – Promo code BrdU-DD4:
Buy one vial of FITC conjugated Mouse Anti-BrdU Antibody (MCA2483FA, 50 µg) and get one vial of Alexa Fluor 488 conjugated Rat Anti-BrdU Antibody (MCA2060A488, 100 tests/1ml) for free

Buy MCA2483FA

* Terms and Conditions
 

  • Promotion is only valid for the anti-BrdU antibody products listed in the four anti-BrdU antibody double deals. All other anti-BrdU antibodies and kits are excluded from the promotion. Bulk and custom anti-BrdU antibody orders are also excluded.
  • Offer applies only to research customers in the following countries and must be placed directly online at bio-rad-antibodies.com or with your local Bio-Rad office: Austria, Belgium, Denmark, Finland, France, Germany, Iceland, Ireland, Luxembourg, The Netherlands, Norway, Sweden, Switzerland, United Kingdom, and United States
  • To qualify for the promotion, promotion codes BrdU-DD1, BrdU-DD2, BrdU-DD3, or BrdU-DD4, must be quoted when placing the order on- or offline
  • Offer expires June 30, 2017
  • Bio-Rad reserves the right to alter the terms and conditions of the promotion or withdraw it at any time
  • Offer excludes shipping costs
  • Offer cannot be combined with any other promotional offers or discounts

References: 
 

  • Aten JA et al. (1992). DNA double labelling with IdUrd and CldUrd for spatial and temporal analysis of cell proliferation and DNA replication. Histochemical Journal 24, 251-259.                                                    
  • Liboska R et al. (2012). Most anti-BrdU antibodies react with 2'deoxy-5'-ethynyluridine - the method for the effective suppression of this cross-reactivity. PLoS One 7, e51679.                                                
  • Kitao H et al. (2016). The antibodies against 5-bromo-2'-deoxyuridine specifically recognize trifluridine incorporated into DNA. Sci Rep 6, 25286.