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Blue Fluorescent Protein Antibody

Blue Fluorescent Protein Antibody gallery image 1

Western blot analysis of 250 ng (lane 1) and 1 μg (lane 2) recombinant BFP probed with Rabbit anti BFP antibody

  • Blue Fluorescent Protein Antibody thumbnail image 1
  • Rabbit anti Blue Fluorescent Protein
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2985WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti BFP antibody recognizes the blue fluorescent protein (BFP), a monomeric engineered derivate of green fluorescent protein (GFP) isolated from the jellyfish Aequorea victoria. BFP is a ~29 kDa protein that is optimally excited at a wavelength of 380 nm and emits light at 448 nm (Day and Davidson 2009). In multi-color and FRET (Fluorescence/Förster Resonance Energy Transfer) experiments, BFP is often used in combination with EGFP (enhanced green fluorescent protein) (Day and Davidson 2009).
    • Intended Use
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to blue fluorescent protein (BFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      30% Glycerol
      0.5% Bovine Serum Albumin
      0.01% Thiomersal
      5mM EDTA
    • Immunogen
      E. coli expressed recombinant blue fluorescent protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti BFP antibody is predicted to recognize a band of approximately 29 kDa on recombinant BFP. Observed band size is 29-36 kDa.
    • Instructions For Use

    Additional Blue Fluorescent Protein Antibody Formats

    Formats Applications Sizes available
    Blue Fluorescent Protein Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti mCherryAHP232650 µgWB
          Rabbit anti Yellow Fluorescent ProteinAHP298350 µgWB
          Rabbit anti Green Fluorescent ProteinAHP298450 µgWB
          Rabbit anti Cyan Fluorescent ProteinAHP298650 µgWB
          Rabbit anti Red Fluorescent ProteinAHP298750 µgWB
          Mouse anti mCherryMCA602050 µgWB

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Blue Fluorescent Protein Antibody thumbnail image 1

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