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Top antibodies for oncoprotein research

Bio-Rad
Aug 13, 2015

Bio-Rad offers a variety of antibodies and proteins for analyzing oncoproteins. For your convenience, we have grouped our oncoprotein research reagents into the four main oncoprotein classes (cell signaling proteins, growth factors, growth factor receptors and transcription factors) and highlighted our highly trusted anti-Bcl-2 and anti-H-Ras antibodies.

Featured murine anti-Bcl-2 antibody (MCA1550)

Featured murine anti-Bcl-2 antibody (MCA1550)
Western blot analysis of a Jurkat cell lysate with mouse anti-human Bcl-2 antibody (MCA1550) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by Chemiluminescence.

Applications: Flow Cytometry, Immunohistochemistry Frozen, Immunohistochemistry Paraffin, Immunoprecipitation and Western Blotting View our application resources

Citations: 11 View all references

The apoptosis regulator Bcl-2 oncoprotein plays an important role in the decision of whether a cell lives or dies. It acts by controlling the permeability of the outer mitochondrial membrane.

Murine anti-Bcl-2 antibody (clone 100) is frequently used in Immunohistochemistry experiments and has been successfully used to demonstrate the presence of Bcl-2 in tissues of radioresistant squamous cell carcinoma (Condon et al. 2002).

Western blot analysis of Bcl-2 is also frequently performed as part of a large apoptosis panel (including other markers such as BakBax and Bcl-xL).

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Featured murine anti-H-Ras antibody (MCA2884)

Western blot analysis of a HEK293 cell lysate with murine anti-human H-Ras antibody (MCA2884) followed by detection with a goat anti-mouse HRP conjugated secondary antibody. Visualization was performed by Chemiluminescence.

Applications: ELISA and Western Blotting  View our application resources

Citations: 2 View all references

The Ras protein family encompasses three members HRAS, NRAS and KRAS.

The genes encoding these proteins present some of the best studied proto-oncogenes, which upon mutation are involved in inducing various cancer types (including bladder, cervical, pancreatic and prostate).

Murine anti-human H-Ras antibody (MCA2884) is frequently used in Western blotting experiments and has been successfully used for the detection of H-Ras in human epitheliod breast cancer cells (MCF10A) (Vuoriluoto et al. 2011).

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References

 

  • Condon LT et al. (2002). Overexpression of Bcl-2 in squamous cell carcinoma of the larynx: a marker of radioresistance. Int J Cancer. 2002 Aug 1;100(4):472-5.
  • Vuoriluoto K et al. (2011). Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer. Oncogene. 2011 Mar 24;30(12):1436-48. 

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