alamarBlue provides a rapid and sensitive way to measure cell proliferation. Just add and measure.
alamarBlue assays are a convenient and quick way to measure cell proliferation and cytotoxicity in human and animal cell lines, bacteria and fungi. Key advantages over traditional MTT and XTT assays include:
Immunohistochemical staining of paraffin embedded mouse colon with Rat anti-BrdU antibody (MCA2060, dark brown). Sections were counterstained with Hematoxylin (blue).
Rat anti-BrdU antibody [BU1/75 (ICR1)] is one of the most trusted and frequently used anti-BrdU antibodies available. The product is supported by a range of comprehensive protocols for use in Immunohistochemistry and Flow Cytometry, and over 50 publications.
Staining of MCF-7 breast cancer cells with the HuCAL anti Ki-67 bivalent Fab antibody (HCA006, green). Nuclei were counterstained with Hoechst (blue) and F-actin with Phalloidin (red).
The anti-Ki-67 antibody (HCA006) is a recombinant bivalent Fab antibody selected from the HuCAL GOLD® phage display library for its specificity against the GFKELFQ epitope, which is present eight times in the human Ki-67 protein. For easy detection the Fab fragment is tagged with myc and His protein tags. Due to the absence of an Fc region, HCA006 is highly recommended for the detection of Ki-67 in cells and tissues that are rich in Fc receptors.
alamarBlue® is manufactured for Bio-Rad by Trek Diagnostic System. US. patent 5,501,959. alamarBlue® is a registered trademark of Trek Diagnostic Systems, Inc.
Apo-BrdUTM is a registered trademark of Phoenix Flow Systems USA.
HuCAL® and HuCAL® GOLD are registered trademarks of MorphoSys AG.
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