Bevacizumab Antibody | AbD17976_hIgG1

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Human Anti-Bevacizumab Antibody specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, HRP conjugated Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 (HCA185) was added at a concentration of 2 µg/ml. Detection was performed using QuantaBlu Fluorogenic Peroxidase Substrate.

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Human Anti-Bevacizumab Antibody bridging ELISA for Anti-Drug Antibody (ADA) assay development

A microtiter plate was coated overnight with bevacizumab at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Bevacizumab Antibody clone AbD17976_hIgG1 (HCA185) in PBST + 10% Human Serum was added. Detection was performed using HRP conjugated bevacizumab at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.

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  • Human anti Bevacizumab
  • Human anti Bevacizumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-bevacizumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human immunoglobulin format; it is suitable as a reference standard in an immune response assay to measure anti-drug antibody levels for bevacizumab or biosimilars.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD17976_hIgG1
    • Isotype
      IgG1
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA185Edatasheet pdfdatasheet pdf0.1 mg
      HCA185
      HCA185GEdatasheet pdfdatasheet pdf1 mg
      HCA185G
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-Bevacizumab Antibody, clone AbD17976_hIgG1 is an affinity-matured, inhibitory, anti-idiotypic antibody that specifically recognizes the humanized monoclonal antibody drug bevacizumab and biosimilar products. This antibody is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay.

        Bevacizumab (reference product branded as Avastin) is a humanized monoclonal antibody (IgG1/kappa) that inhibits angiogenesis by binding to VEGF-A. VEGF-A is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. Bevacizumab is used to treat various cancers, including colorectal, lung, breast, glioblastoma, kidney and ovarian.

        View a summary of all Anti-Bevacizumab Antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Bevacizumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.4 nM by real time, label-free molecular interaction analysis on immobilized bevacizumab.
      • Approx. Protein Concentrations
        Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
        Pack Size: 1 mgTotal protein concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
        Pack Size: 1 mgTotal protein concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Avastin is a trademark of Genentech Inc.
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Avastin is a trademark of Genentech Inc.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product is suitable for use in direct ELISA applications.
        This antibody is fully human and can be used as a reference standard in an ADA assay.
        Protocol: ADA bridging ELISA
      • ELISA
        This product is suitable for use in direct ELISA applications.
        This antibody is fully human and can be used as a reference standard in an ADA assay.
        Protocol: ADA bridging ELISA
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Bevacizumab Antibody Formats

      Formats Clone Applications Sizes available
      Bevacizumab Antibody : Purified AbD17976_hIgG1 E 0.1 mg | 1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti BevacizumabHCA1820.1 mgE
            HCA182
            Human anti BevacizumabHCA1840.1 mgE
            HCA184
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK001P1 Conjugation For 400µg AntibodyCJ
            LNK001P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti BevacizumabHCA1820.1 mgE
            HCA182
            Human anti BevacizumabHCA1840.1 mgE
            HCA184

            Recommended Positive Controls

              Histology Controls

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