Staphylococcus aureus Antibody | Staph11-232.3

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Staphylococcus aureus Antibody | Staph11-232.3 gallery image 1

Published customer image:
Mouse anti Staphylococcus aureus antibody, clone Staph11-232.3 (MCA5792) used as a capture reagent in an ELISA to evaluate linear peptide binding.
Image caption:
Both SP27 and MAP27' bind to anti-PGN mAb specifically. (A) The sequences of SP27 (Biotin-SASPHHHSRLRSESGG) and SP27' (Biotin-SPHHHSRLRSESSAGG). Underlined letters represent nonspecific flanking amino acids. (B) Both SP27 and SP27' bind to anti-PGN mAb in a dose-dependent manner. (C) Both SP27 and SP27' (100 µg/ml) specifically bind to anti-PGN mAb, but not to anti-LTA mAb. For ELISA assays in panel B and C, anti-PGN mAb or anti-LTA mAb was used to coat the wells at a concentration of 5 µg/ml, SP27, SP27' or non-specific peptide L2 (in panel B, sequence: Biotin-HSGHWDFRQWWQPSGG) was then added at indicated concentrations and incubated at 37°C for 40 min, followed by detection with HRP-labeled streptavidin. (D) The structure diagram of MAP27. MAP27 was synthesized in a tetra-branched form that contains four copies of a sequence (SASPHHHSRLRSESGG) that mimics PGN epitope. (E) MAP27 binds to anti-PGN mAb specifically. (F) MAP27 binds to anti-S. aureus polyclonal antibodies specifically. For ELISA assays in panel E and F, MAP27 or MAPctrl was used to coat the wells of a microplate. Anti-PGN mAb or anti-S. aureus polyclonal antibodies was added, followed by detection with HRP-labeled antibodies. The absorbance was measured at OD450nm. The results are shown as means ±SEM. * P<0.05, ** P<0.01, *** P<0.001.

From: Wang X-Y, Huang Z-X, Chen Y-G, Lu X, Zhu P, Wen K, et al. (2015) A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.
PLoS ONE 10(8): e0136888.

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  • Mouse anti Staphylococcus aureus ATCC 29740
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  • Product Type
    Monoclonal Antibody
  • Clone
    Staph11-232.3
  • Isotype
    IgG3
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5792Edatasheet pdfdatasheet pdf0.2 mg
    MCA5792
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 recognizes the peptidoglycan of S. aureus, protein A-negative S. aureus and S. epidermidis.

      Mouse anti Staphylococcus aureus ATCC 29740 antibody, clone Staph11-232.3 does not cross-react with Streptococcus sp., Mycoplasma sp. or Cornybacteria sp., however it has not been cross-adsorbed and may react with peptidoglycans from other related species.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG3 prepared by affinity chromatography from ascites
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      UV-inactivated Staphylococcus aureus cells (ATCC 29740).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/5000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Staphylococcus aureus Antibody Formats

    Formats Clone Applications Sizes available
    Staphylococcus aureus Antibody : Purified Staph11-232.3 E 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

              Further Reading

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