Salmonella paratyphi A LPS Antibody | 10B10G

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  • Mouse anti Salmonella paratyphi A LPS Group A 0-2 Antigen
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  • Product Type
    Monoclonal Antibody
  • Clone
    10B10G
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2831E, IB, IFdatasheet pdfdatasheet pdf0.2 mg
    MCA2831
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Salmonella paratyphi A LPS Group A 0-2 Antigen antibody, clone 10B10G recognizes the O antigen of Salmonella enterica, serovar paratyphi A (also known as S. paratyphi). The O antigen is a polysaccharide that extends from the core oligosaccharide, which itself is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs. The O antigen is the outermost component of the LPS and functions as a receptor for bacteriophages. It is also important in the immune response of the host. Considerable diversity is seen within Salmonella O antigens.

      Salmonella enterica, serovar paratyphi A belongs to serogroup A of Salmonella and causes paratyphoid fever, which is similar to typhoid fever but more benign.

      Mouse anti Salmonella paratyphi A LPS Group A 0-2 Antigen antibody, clone 10B10G recognizes the 0-2 antigenic determinant of Salmonella serogroup A and does not cross-react with the LPS of serogroups B, D or E of Salmonella spp.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Heat inactivated cells of Salmonella paratyphi A.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of Sp2/0 the myeloma cells.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoblotting
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Salmonella paratyphi A LPS Antibody Formats

    Formats Clone Applications Sizes available
    Salmonella paratyphi A LPS Antibody : Purified 10B10G E, IB, IF 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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