Salmonella Antibody | 5D12A

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  • Mouse anti Salmonella Broad Reactivity
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5D12A
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2832E, IB, IFdatasheet pdfdatasheet pdf0.2 mg
    MCA2832
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A recognizes the core antigen of Salmonella enterica, serogroups A, B, C1, C2, D, E1 and E2. The core antigen bears the O antigen and is attached to lipid A. These three components together form the lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria. The LPS is an endotoxin and induces a strong immune response from the host. Its mutation or removal results in the death of the bacterial cell and as such is a prime target for antimicrobial drugs.

      Salmonella enterica,causes typhoid fever, paratyphoid fever and foodborne illnesses.

      Mouse anti Salmonella Broad Reactivity antibody, clone 5D12A cross-reacts with serogroup A (S. paratyphi A), serogroup B (S. typhimurium), serogroup C1 (S. choleraesuis.), serogroup C2, (S. newport), serogroup D (S. enteriditis), serogroup E1 (S. anatum) and serogroup E2 (S. selandia). It does not cross-react with Escherichia coli 055:B5, E. coli K12 or Klebsiella pneumoniae.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Heat inactivated Salmonella paratyphi.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the NS0/1, a subclone of the P3X63Ag8 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoblotting
      Immunofluorescence

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Salmonella Antibody Formats

    Formats Clone Applications Sizes available
    Salmonella Antibody : Purified 5D12A E, IB, IF 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              Source Reference

              Antibody Characterization Reference

              References

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