Protein G

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  • Recombinant Protein G
  • Recombinant Protein G:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    740605Edatasheet pdfdatasheet pdf1 ml
    740605
    740601LE, IPdatasheet pdfdatasheet pdf5 mg
    740601L
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Protein G is a preparation of streptococcal protein G expressed in E. coli. Protein G binds to the Fc portion of immunoglobulin G subclasses (IgGs) from a broad range of species including human and mouse, and does not bind IgA, IgE, IgM or serum albumin.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Recombinant Protein - lyophilised
      Recombinant protein - conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
      Reconstitute with 1ml phosphate buffered saline
    • Preparation
      Recombinant Protein G, from Strepococcus strain G148, expressed in E.coli
      Recombinant protein G from Streptococcus strain G148, expressed in E.coli.
    • Preservative Stabilisers
      None present
      50% Glycerol
    • Purity
    • Approx. Protein Concentrations
    • Molecular Weight
      22kDa (monomeric protein lacking cystine residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      6 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/40001/8000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Protein G Formats

    Formats Applications Sizes available
    Protein G : Rec. Protein E, IP 5 mg
    Protein G : HRP E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A

          Recommended Positive Controls

            Histology Controls

              References

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