Listeria monocytogenes Antibody | LZF7 (BGN/0884/67)

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  • Mouse anti Listeria monocytogenes
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LZF7 (BGN/0884/67)
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0400-0027E, WBdatasheet pdfdatasheet pdf0.2 mg
    0400-0027
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Listeria monocytogenes antibody, clone LZF7 recognizes both intact Listeria sp and outer membrane preparations reacting with a ~37 kDa protein in western blotting. Mouse anti Listeria monocytogenes antibody, clone LZF7 recognizes strains of Listeria monocytogenes including ATCC 19114, LMG 16780 and LMG 23190.

      Mouse anti Listeria monocytogenes antibody, clone LZF7 has also been shown to cross-react with other Listeria species including L. innocua ATCC 33090, L. ivanovii ATCC 19119 and L. welshimeri ATCC 3589.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Listeria monocytogenes, outer membrane fraction.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product is suitable for use as a detection antibody in a sandwich ELISA, with 0400-0030 as the coating antibody. Please note, the activity of this antibody is reduced on boiling of the bacteria.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      0400-0027 detects a band of approximately 23kDa in reduced blots of cell fragments.
    • Instructions For Use

    Additional Listeria monocytogenes Antibody Formats

    Formats Clone Applications Sizes available
    Listeria monocytogenes Antibody : Purified LZF7 (BGN/0884/67) E, WB 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Listeria monocytogenes0400-00300.2 mgE, WB
          0400-0030

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Surface Plasmon Resonance

              References

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