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Listeria monocytogenes antibody

Rabbit anti Listeria monocytogenes Kc 1778+ H 2b

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Listeria monocytogenes

Product Code Applications Pack Size List Price Your Price Qty
5688-1990
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * 1 ml loader
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Rabbit anti Listeria monocytogenes antibody reacts with Listeria monocytogenes whole cells. Rabbit anti Listeria monocytogenes antibody has not been absorbed and may react with related micro-organisms.

Rabbit anti Listeria monocytogenes antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015).

Target Species
Bacterial
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Listeria monocytogenes. whole cells.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Listeria monocytogenes antibody

  1. Témoin, S. et al. (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains.
    Microbiology. 154 (Pt 3): 939-48.
  2. Marusov, G. et al. (2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.
    Environ Sci Technol. 46 (1): 348-59.
  3. Salem M et al. (2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.
    Clin Exp Immunol. 179 (3): 426-34.
  4. Okita, Y. et al. (2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells against Listeria monocytogenes.
    Infect Immun. 84 (2): 573-9.
  5. Cruz-Adalia, A. et al. (2017) Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses.
    Nat Commun. 8 (1): 1591.
  6. García-Ferreras, R. et al. (2023) Bacteria-instructed B cells cross-prime naïve CD8(+) T cells triggering effective cytotoxic responses.
    EMBO Rep. 24 (7): e56131.

Flow Cytometry

Immunofluorescence

RRID
AB_620691

5688-1990

151444 155299 156141 159516

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