Flavobacterium Psychrophilum Antibody | FL43

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  • Mouse anti Flavobacterium Psychrophilum:FITC
  • Mouse anti Flavobacterium Psychrophilum:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    FL43
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5978FIFdatasheet pdfdatasheet pdf0.1 mg
    MCA5978F
    MCA5978PE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5978P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Flavobacterium psychrophilum, clone FL43, specifically recognizes the infectious bacteria Flavobacterium psychrophilum, previously designated as Cytophaga psychrophila and Flexibacter psychrophilus. F. pschrophillum is the causative agent of fish disease known under a range names, most commonly as Bacterial cold water disease (BCWD) in America and Rainbow trout fry syndrome (RTFS) in Europe. F. psychrophilum has a worldwide distribution where it is responsible for serious disease outbreaks in predominantly freshwater salmonid fish farms and hatcheries resulting in significant ecconomic loss for the aquaculture industry.

      While F. pyschrophilum infections are primarily in young fish, with Rainbow trout and Coho salmon being particularly susceptable, salmonids of all ages are subject to infection. Additionally F. pyschrophilum has been detected in a range of non salmonids including species from eel, (Anguilla sp.), and carp (Carassius sp.) families (Barnes, M.E., et al. 2011).

      While the route of infection is not fully understood, it is beleived that infection may in part be from parent to offspring, however it is known that F. psychrophilum survives outside its host in an aquatic environment for a considerable time period. Additionally other fish, both inter- and intraspecies can function as a reservoir for infection, particulary dying and dead individuals that have been demonstrated to shed increased levels of bacteria (Madetoja, J., et al. 2000). Infection results in a range of clinical symptoms including necrosis of tissues in such as the fins, enlarged organs, in particular, the spleen, neurological damage and morphological deformities. Mortality varies widely from 10% to over 75% and it is believed that the variation in mortality may be affected by a combination of factors including, but not limited to, bacterial strain virulence, water temperature, genetic diversity within fish stocks and stress (Barnes, M.E., et al. 2011).

      Clone FL43 has been shown to be specific for F.psychrophilum and does not recognise strains of the closely related F. columnare, F. pectinovorum, F. aquatile, F. branchiophilum, and F. saccharophilumte tested to date. Clone FL43 may therefore be used to identify F. psycophilum in tissues of infected individuals and may be of use in research to develop treatment regimes for farmed salmonid species (Lindstrom, N.M., et al. 2009) .
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.01% Gentamicine sulphate
      1% Bovine Serum Albumin
    • Immunogen
      Outer membrane fractions of F. psychrophilum (wildtype strain CSF 259-95)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.73 mg/ml
      IgG concentration 2.31 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Mouse X63-Ag8.6.5.3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Flavobacterium Psychrophilum Antibody Formats

    Formats Clone Applications Sizes available
    Flavobacterium Psychrophilum Antibody : FITC FL43 IF 0.1 mg
    Flavobacterium Psychrophilum Antibody : HRP FL43 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Salmonid IgMCA21820.5 mlE, F, IF, WB
          MCA2182
          Mouse anti Rainbow Trout IgMCA59760.5 mlC, E, IF, WB
          MCA5976
          Rabbit anti Salmonid IgAHP76150 µlE, IF, WB
          AHP761
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Salmonid IgMCA21820.5 mlE, F, IF, WB
          MCA2182
          Mouse anti Rainbow Trout IgMCA59760.5 mlC, E, IF, WB
          MCA5976
          Rabbit anti Salmonid IgAHP76150 µlE, IF, WB
          AHP761

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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