Cholera Toxin Antibody | 2/166

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  • Mouse anti Cholera Toxin Alpha
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  • Product Type
    Monoclonal Antibody
  • Clone
    2/166
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9540-0008Edatasheet pdfdatasheet pdf0.2 mg
    9540-0008
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Cholera Toxin alpha antibody, clone 2/166 recognizes the alpha subunit of Cholera toxin.

      The cholera holotoxin consists of a pentameric ring of Beta subunits whose central pore is occupied by the Alpha subunit. The Alpha subunit contains two chains, A1 and A2, linked by a disulfide bridge. The Alpha subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Vibrio cholerae extract
    • Purity
    • Approx. Protein Concentrations
      5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      pathogenesis
      extracellular region
      protein ADP-ribosylation
      NAD+-diphthamide ADP-ribosyltransferase activity
      NAD+ ADP-ribosyltransferase activity
      killing of cells of other organism
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Cholera Toxin Antibody Formats

    Formats Clone Applications Sizes available
    Cholera Toxin Antibody : Purified 2/166 E 0.2 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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