Chlamydia trachomatis LPS antibody | 1312/236
Mouse anti Chlamydia trachomatis LPS antibody, clone 1312/236 (1990-2809) used for the identification of Chlamydia lipopolysaccharide by western blotting.
Image caption:
Western blot analysis of small bowel biopsies from 4 IBS patients against mouse immune sera to LPS and MOMP, respectively. 1-4 = IBS patients, ++ = HeLa cells infected with C. trachomatis (10 μl), + = HeLa cells infected with C. trachomatis (5 μl), - = non-infected HeLa cells.
From: Dlugosz A, T&oull;rnblom H, Mohammadian G, Morgan G, Veress B, Edvinsson B, Sandström G, Lindberg G. Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome.
BMC Gastroenterol. 2010 Feb 16;10:19
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Chlamydia trachomatis LPS
- Product Type
- Monoclonal Antibody
- Clone
- 1312/236
- Isotype
- IgG1
- Specificity
- Chlamydia trachomatis LPS
Antibody Quality and Performance Guarantee
| Mouse anti Chlamydia trachomatis LPS, clone 1312/236 recognizes C. trachomiatis lipopolysaccharide. In a simple ELISA this antibody is reactive with 15 serovars of C. trachomatis. The epitope has been shown by Western blotting to be present in LPS. |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Native.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for Chlamydia trachomatis LPS antibody
-
Dlugosz, A. et al. (2010) Chlamydia trachomatis antigens in enteroendocrine cells and macrophages of the small bowel in patients with severe irritable bowel syndrome.
BMC Gastroenterol.10:19. -
Mosolygó, T. et al. (2013) IL-17E production is elevated in the lungs of Balb/c mice in the later stages of Chlamydia muridarum infection and re-infection.
In Vivo. 27 (6): 787-92. -
Bogdanov, A. et al. (2014) Application of DNA chip scanning technology for automatic detection of Chlamydia trachomatis and Chlamydia pneumoniae inclusions.
Antimicrob Agents Chemother. 58 (1): 405-13. -
Mosolygó, T. et al. (2014) Expression of Chlamydia muridarum plasmid genes and immunogenicity of pGP3 and pGP4 in different mouse strains.
Int J Med Microbiol. 304 (3-4): 476-83. -
Balogh, E.P. et al. (2014) Anti-chlamydial effect of plant peptides.
Acta Microbiol Immunol Hung. 61 (2): 229-39. -
Mosolygó T et al. (2014) Protection promoted by pGP3 or pGP4 against Chlamydia muridarum is mediated by CD4(+) cells in C57BL/6N mice.
Vaccine. 32 (40): 5228-33.
- RRID
- AB_618921
1990-2809
149917
154987
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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