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Chlamydia trachomatis

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  • Product Type
    Antigen
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MPP014Edatasheet pdfdatasheet pdf1 ml
    MPP014
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Chlamydia trachomatis is a non-motile, Gram-negative human pathogen. It undergoes a unique biphasic developmental cycle, forming elementary bodies extracellularly and distinctive intracellular inclusions. The elementary body is infectious but metabolically inactive, cannot replicate and lacks detectable peptidoglycan.

      Chlamydia trachomatis is a common sexually transmitted bacterium. Chlamydia infection may cause proctitis, urethritis, trachoma (an eye infection and the leading cause of blindness worldwide) and infertility in both sexes. Prostatitis and epididymitis may occur in men and in women infection might lead to acute or chronic pelvic pain, pelvic inflammatory disease, cervicitis and ectopic pregnancy. In neonates the pathogen may cause trachoma and pulmonary complications.

      MPP014 is a heat inactivated saline lysate preparation of Chlamydia trachomatis. This preparation is useful for applications that require low concentrations of contaminating protein.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Inactivated Chlamydia trachomatis, LGV type II strain 434, saline lysate - frozen
    • Reconstitution
    • Preparation
      Optimally infected cells are washed and disrupted into a small volume of phosphate buffered saline, subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris.

      The preparation is inactivated by heat treatment and 0.05% Triton X-100. Inactivation is verified by two blind passages on permissive cells showing no inclusion bodies.
    • Preservative Stabilisers
      None present
    • Activity
      This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 4.2mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      0.05% Triton X-100
    • Storage
      Store at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Chlamydia trachomatis Formats

    Formats Applications Sizes available
    Chlamydia trachomatis : Inactivated Pathogen E 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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