Negative Isotype Controls
- Chlamydia trachomatis is a non-motile, Gram-negative human pathogen. It undergoes a unique biphasic developmental cycle, forming elementary bodies extracellularly and distinctive intracellular inclusions. The elementary body is infectious but metabolically inactive, cannot replicate and lacks detectable peptidoglycan.
Chlamydia trachomatis is a common sexually transmitted bacterium. Chlamydia infection may cause proctitis, urethritis, trachoma (an eye infection and the leading cause of blindness worldwide) and infertility in both sexes. Prostatitis and epididymitis may occur in men and in women infection might lead to acute or chronic pelvic pain, pelvic inflammatory disease, cervicitis and ectopic pregnancy. In neonates the pathogen may cause trachoma and pulmonary complications.
MPP014 is a heat inactivated saline lysate preparation of Chlamydia trachomatis. This preparation is useful for applications that require low concentrations of contaminating protein.
Inactivated Chlamydia trachomatis, LGV type II strain 434, saline lysate - frozen
Optimally infected cells are washed and disrupted into a small volume of phosphate buffered saline, subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris.
The preparation is inactivated by heat treatment and 0.05% Triton X-100. Inactivation is verified by two blind passages on permissive cells showing no inclusion bodies.
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 4.2mg/ml.
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
0.05% Triton X-100
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
18 months from date of despatch.
For research purposes only
Additional Chlamydia trachomatis Formats
Recommended Secondary Antibody
Recommended Negative Isotype Control
Recommended Positive Controls