Borrelia burgdorferi is a spirochete, the causitive agent of the tick borne zoonotic disease Borreliosis or Lyme disease. The organism was first isolated in 1982 by Willy Burgdorfer after whom the organism was named. B. burgdorferi is the principal cause of Lyme disease in the U.S while B. afzelii and B. garinii are found as causitive agents in Europe.
Sonicated organism suspension of B. burgdorferi (strain B31) - liquid
Rendered inactive using gamma irradiation. The source was cultured in BSK II medium and the preparation contains all major antigens with residual cellular debris. The preparation was considered inactive when no detection of growth in two blind passages under original culture conditions is observed
1440-0006 was prepared from B. burgdorferi strain B31 grown in BSK11 medium.
Approx. Protein Concentrations
0.90 mg/ml using the Biorad dye binding assay. Standard curve is generated with a known concentration of IgG.
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -70oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
12 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Instructions For Use
This preparation should be sonicated immediately prior to use to ensure that the preparation is uniform.
1. Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.Fluids Barriers CNS. 9: 5.