Beta Galactosidase Antibody

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Published customer image:
Rabbit anti beta glalctosidase antibody (AHP1292GA) used for the demonstration of β-glalacosidase expressing cells in embryonic mouse tissues by immunofluorescence.
Image caption:
MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and ß-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 µm in A and G, and 50 µm in E.

From: Wende C-Z, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.
PLoS ONE 10(5): e0127681.

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  • Rabbit anti Beta Galactosidase
  • Rabbit anti Beta Galactosidase:FITC
  • Rabbit anti Beta Galactosidase:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1292GAC, E, P, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1292GA
    AHP1292FAIFdatasheet pdfdatasheet pdf1 mg
    AHP1292FA
    AHP1292BBC, E, P, WBdatasheet pdfdatasheet pdf1 mg
    AHP1292BB
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides.

      Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.01% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.01% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Beta-galactosidase prepared from E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 5.0 mg/ml
      IgG concentration 10 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      lactose catabolic process
      beta-galactosidase complex
      carbohydrate binding
      metal ion binding
      beta-galactosidase activity
      protein binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/4001/1600
      Immunohistology - Frozen1/501/300
      Immunohistology - Paraffin1/501/300
      Western Blotting1/2001/500
    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/100
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/100001/50000
      Immunohistology - Frozen1/10001/5000
      Immunohistology - Paraffin1/10001/5000
      Western Blotting1/20001/10000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP1292GA detects a band of approximately 117kDa using E. coli beta-galactosidase.
    • Western Blotting
    • Western Blotting
      AHP1292BB detects a band of approximately 117kDa using E. coli beta-galactosidase.
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Beta Galactosidase Antibody Formats

    Formats Applications Sizes available
    Beta Galactosidase Antibody : Biotin C, E, P, WB 1 mg
    Beta Galactosidase Antibody : FITC IF 1 mg
    Beta Galactosidase Antibody : Purified C, E, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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