Beta Galactosidase Antibody

Beta Galactosidase Antibody gallery image 1

Published customer image:
Rabbit anti beta glalctosidase antibody (AHP1292GA) used for the demonstration of β-glalacosidase expressing cells in embryonic mouse tissues by immunofluorescence.
Image caption:
MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and ß-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 µm in A and G, and 50 µm in E.

From: Wende C-Z, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.
PLoS ONE 10(5): e0127681.

  • Beta Galactosidase Antibody thumbnail image 1
  • Rabbit anti Beta Galactosidase
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1292GAC, E, P, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides.

      Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Beta-galactosidase prepared from E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      lactose catabolic process
      beta-galactosidase complex
      carbohydrate binding
      metal ion binding
      beta-galactosidase activity
      protein binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/501/300
      Immunohistology - Paraffin1/501/300
      Western Blotting1/2001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1292GA detects a band of approximately 117kDa using E. coli beta-galactosidase.
    • Instructions For Use

    Additional Beta Galactosidase Antibody Formats

    Formats Applications Sizes available
    Beta Galactosidase Antibody : Purified C, E, P, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls


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