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Beta Galactosidase Antibody | 2E9
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Enlarge- Mouse anti Beta Galactosidase
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Product TypeMonoclonal Antibody
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Clone2E9
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti beta Galactosidase antibody, clone 2E9 reacts with beta-galactosidase from E. coli and Aspergillus oryzae. Less than 10% reactivity is observed with galactosidase from bovine liver. This antibody does not influence enzyme activity.
Beta-galactosidase is an enzyme that acts to catalyse the hydrolysis of beta-galactosides into monosaccharides.
Beta-galactosidase is a homo-tetrameric protein, containing four identical sub-units of approximately 116 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to determine the success of transfections.
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Intended Use
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Target SpeciesBacterial
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Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
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ImmunogenPurified beta-galactosidase from E. coli.
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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Fusion PartnersSpleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termsmetal ion bindingbeta-galactosidase activitycarbohydrate bindingbeta-galactosidase complexlactose catabolic processprotein binding
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UniProt
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional Beta Galactosidase Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| Beta Galactosidase Antibody : Purified | 2E9 | E | 0.2 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
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