Beta Galactosidase Antibody | 2E9

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  • Mouse anti Beta Galactosidase
  • Mouse anti Beta Galactosidase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2E9
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5785Edatasheet pdfdatasheet pdf0.2 mg
    MCA5785
    MCA5785GEdatasheet pdfdatasheet pdf1 mg
    MCA5785G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti beta Galactosidase antibody, clone 2E9 reacts with beta-galactosidase from E. coli and Aspergillus oryzae. Less than 10% reactivity is observed with galactosidase from bovine liver. This antibody does not influence enzyme activity.

      Beta-galactosidase is an enzyme that acts to catalyse the hydrolysis of beta-galactosides into monosaccharides.

      Beta-galactosidase is a homo-tetrameric protein, containing four identical sub-units of approximately 116 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to determine the success of transfections.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      Pack Size: 0.2 mg0.09% Sodium Azide (NaN3)
      Pack Size: 1 mg0.1% Sodium Azide (NaN3)
      Pack Size: 0.2 mg0.09% Sodium Azide (NaN3)
      Pack Size: 1 mg0.1% Sodium Azide (NaN3)
    • Immunogen
      Purified beta-galactosidase from E. coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      metal ion binding
      beta-galactosidase activity
      carbohydrate binding
      beta-galactosidase complex
      lactose catabolic process
      protein binding
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Beta Galactosidase Antibody Formats

    Formats Clone Applications Sizes available
    Beta Galactosidase Antibody : Purified 2E9 E 1 mg | 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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