Apoptosis TUNEL Assay IHC Kit

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Measurement of apoptotic changes in the lung after ricin intoxication. Apoptotic staining (TUNEL, green; DAPI, blue) in naive (left) and non-labeled-ricin-intoxicated mice (six mice per group). Scale bars indicate 20 μm.

From: Sapoznikov, A. et al.Diverse Profiles of Ricin-Cell Interactions in the Lung Following Intranasal Exposure to Ricin. Toxins 2015, 7(11), 4817-31.

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Apoptosis TUNEL Assay IHC Kit gallery image 2

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Immunofluorescence analysis of ATII cells after ricin exposure. (A) Co-immunofluorescence for pro-SPC (red), TUNEL (green) and DAPI (blue) from lung tissue 24 h after intoxication. Arrows point to TUNEL-positive, apoptotic ATII cells; (B) Immunofluorescence for pro-SPC (red) and DAPI (blue) staining of lung tissue from naive (left) and 24 h post-intoxicated (right) mice. Circles indicate pro-SPC+ ring structures; (C) Visualization of bronchiole structures (arrows) stained for pro-SPC (red) and DAPI (blue) in lung tissue from naive (left) and 24 h post-intoxicated (right) mice. Scale bars indicate 20 μm.

From: Sapoznikov, A. et al.Diverse Profiles of Ricin-Cell Interactions in the Lung Following Intranasal Exposure to Ricin. Toxins 2015, 7(11), 4817-31.

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  • APO-BrdU-IHC™ Immunohistochemistry Kit
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  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    APO002C, Pdatasheet pdfdatasheet pdf50 Tests
    APO002
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • APO-BrdU IHC™ Immunohistochemistry Kit (APO002) is a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) immunohistochemical assay kit, which enables the identification of cells during the late stages of apoptosis, through the labelling of DNA breaks (DNA fragmentation).

      APO-BrdU IHC™ Immunohistochemistry Kit (APO002) can be used for the staining of both tissue cryosections and paraffin-embedded tissue, and also cell preparations fixed on slides.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      1x box 2 Control Slides
      1x 22ml bottle Blocking Buffer
      1x vial 6 H202/Urea Tablets
      1x 110ul vial Proteinase-K
      1x vial 6 DAB Tablets
      1x 41ul vial TdT Enzyme
      1x 440ul vial Br-dUTP
      1X 35ul vial 200X Conjugate
      1x 1.75ml vial 5X Reaction Buffer
      1x 275ul vial Biotin anti-BrdU mAb
      1x 6ml bottle Methyl Green
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC until first use.
      MULTIPLE STORAGE CONDITIONS APPLY THEREAFTER. Please refer to PDF manual.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
      Apo-BrdU-IHC™ is a registered trademark of Phoenix Flow Systems USA
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Three detailed protocols are provided in the instructions for use. Apoptosis measurement of paraffin-embedded tissue sections, cell preparations fixed on slides, and tissue cryosections. The instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/APO002.pdf

    Additional Apoptosis TUNEL Assay IHC Kit Formats

    Formats Applications Sizes available
    Apoptosis TUNEL Assay IHC Kit : Kit C, P 50 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F

          Recommended Positive Controls

            Histology Controls

              References

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