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APO BrdU TUNEL assay Fluorescence Kit (APO001) used for the evaluation of apoptosis in bovine mononuclear cells in vitro by two color flow cytometry.
BRSV lacking the SH gene induces apoptosis in MDBK cells and bovine monocytes. (a) MDBK cells were infected with WT rBRSV or rBRSV lacking the SH gene (ΔSH) at an m.o.i. of 3. (b) Bovine monocytes were infected with WT or ΔSH rBRSV at an m.o.i. of 1 or 3. As a control, cells were exposed to mock-infected tissue culture cell lysate. The proportions of apoptotic cells were determined 24 and 48 h p.i. of MDBK cells, or 24 h p.i. of monocytes, using a TUNEL assay and flow cytometry. Results are expressed as the mean percentage apoptotic cells±sd of triplicate samples. *, P<0.0001; **, P<0.01.
From: Taylor G, Wyld S, Valarcher JF, Guzman E, Thom M, Widdison S, Buchholz UJ.
Recombinant bovine respiratory syncytial virus with deletion of the SH gene induces increased apoptosis and pro-inflammatory cytokines in vitro, and is attenuated and induces protective immunity in calves.
J Gen Virol. 2014 Jun;95(Pt 6):1244-54.