Rubella Virus

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Native Rubella Virus

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP044 E 1 mg
Native Rubella Virus preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE buffer with sucrose. Some residual non-viral protein from the host tissue is present. The preparation does not contain detergent.

Rubella virus is an enveloped, single-stranded RNA virus of the Togaviridae family. Infection with the virus leads to rubella, commonly known as German measles, a common childhood illness. The primary symptom is a rash on the face, trunk and limbs which normally fades after about three days. Fever, swollen glands, joint pains, headaches and conjunctivitis may also be present. Symptoms are usually mild and the virus is rapidly eliminated from the body. Infection of a pregnant woman is potentially serious since the child may be born with congenital rubella syndrome, characterised by cardiac, cerebral, ophthalmic and auditory defects.

For a more highly purified Rubella virus preparation, please see PIP045.

Product Details

Target Species
Viral
Product Form
Inactivated Rubella virus - liquid
Preparation
Vero cells infected with Rubella virus release virus particles into the culture supernatant. The supernatant is harvested, clarified and concentrated. The virus suspension is purified by one cycle of sucrose gradient purification.

The antigen preparation is inactivated by exposure to UV light. This method leads to maximum loss of infectivity with a minimum loss of antigenicity.
Source
Rubella virus, HPV-77 strain, cultured in Vero cells, an established cell line from African Green Monkey kidney.
Buffer Solution
NTE buffer (Sodium chloride, Tris and EDTA) + sucrose buffer
Preservative Stabilisers
None present
Activity
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 1.10 mg/ml.

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Rubella Virus

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
PIP044 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats.