Herpes simplex Virus 2

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Native Herpes simplex Virus 2

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP010 E 1 ml
Herpes simplex virus 2 (HSV-2) belongs to Herpesviridae, a family of viruses with several members that invade the human nervous system, causing a variety of illnesses including cold sores, chickenpox, shingles and various cancers. Herpes viruses may also cause encephalitis or infectious corneal blindness. HSV is transmitted during close contact with an infected person who is shedding virus from the skin, in saliva or in secretions from the genitals and commonly persists in latent infection.

This Native Herpes simplex Virus 2 product contains a high concentration of virus particles and components in tissue culture medium contianing a low concentration of bovine serum. Some residual host cellular material is present.

Product Details

Target Species
Viral
Product Form
Inactivated Herpes simplex virus 2 - liquid
Preparation
Herpes simplex virus 2, MS strain, cultured in BSC-1, a cell line derived from African Green monkey kidney. Herpes simplex virus 2 antigen is collected from both the cell and supernatant phases of tissue culture. The antigen is harvested by disrupting optimally infected monolayers in the tissue culture medium. Cellular debris is removed by centrifugation. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.
Buffer Solution
Tissue culture medium
Preservative Stabilisers
None present
Activity
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 2.22 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Herpes simplex Virus 2

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
PIP010 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.