Herpes simplex Virus 2
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Herpes simplex virus 2 (HSV-2) belongs to Herpesviridae, a family of viruses with several members that invade the human nervous system, causing a variety of illnesses including cold sores, chickenpox, shingles and various cancers. Herpes viruses may also cause encephalitis or infectious corneal blindness. HSV is transmitted during close contact with an infected person who is shedding virus from the skin, in saliva or in secretions from the genitals and commonly persists in latent infection.
This Native Herpes simplex Virus 2 product contains a high concentration of virus particles and components in tissue culture medium contianing a low concentration of bovine serum. Some residual host cellular material is present.
This Native Herpes simplex Virus 2 product contains a high concentration of virus particles and components in tissue culture medium contianing a low concentration of bovine serum. Some residual host cellular material is present.
Product Details
- Target Species
- Viral
- Product Form
- Inactivated Herpes simplex virus 2 - liquid.
- Preparation
- Herpes simplex virus 2 antigen is collected from both the cell and supernatant phases of tissue culture. The antigen is harvested by disrupting optimally infected monolayers in the tissue culture medium. Cellular debris is removed by centrifugation.
The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material. - Source
- Herpes simplex virus 2, MS strain, cultured in BSC-1, a cell line derived from African Green monkey kidney.
- Buffer Solution
- Tissue culture medium
- Preservative Stabilisers
- None present
- Activity
- This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
- Approx. Protein Concentrations
- Total protein concentration 2.22 mg/ml
Storage Information
- Storage
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Herpes simplex Virus 2
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- PIP010 should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.
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