Hepatitis A Virus

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Native Hepatitis A Virus

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PIP008 E 1 ml
Native Hepatitis Virus preparation contains a high concentration of viral antigen, predominantly in the form of whole virions. The preparation also contains a low concentration of formaldehyde with some residual host cellular material.

Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross-react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4.

Hepatitis A is a wide-spread infectious disease, resulting from infection of the liver by the Hepatitis A virus (HAV). Hepatitis A infection causes jaundice, fever, anorexia, nausea, vomiting, headache and fatigue. Some cases may be fatal. The virus is mainly transmitted by the faecal-oral route via contaminated food or drinking water. The HAV is a picornavirus that replicates slowly without noticeable interference to host cell macro-molecular synthesis and, consequently, without apparent cytopathic effect. The liver pathology observed is likely to be immune-mediated.

Product Details

Target Species
Viral
Product Form
Inactivated Hepatitis A virus - liquid
Preparation
Hepatitis A virus, strain HM-175, cultured in FRhK 4 , an established cell line from fetal Rhesus monkey kidney. Cells infected with Hepatitis A virus are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and disrupted to release viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with freon. The antigen preparation is inactivated by incubating with formaldehyde for 96 hours.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
None present
Activity
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 0.30 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Hepatitis A Virus

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
This product should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.