Hepatitis A Virus
Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross-react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4.
Hepatitis A is a wide-spread infectious disease, resulting from infection of the liver by the Hepatitis A virus (HAV). Hepatitis A infection causes jaundice, fever, anorexia, nausea, vomiting, headache and fatigue. Some cases may be fatal. The virus is mainly transmitted by the faecal-oral route via contaminated food or drinking water. The HAV is a picornavirus that replicates slowly without noticeable interference to host cell macro-molecular synthesis and, consequently, without apparent cytopathic effect. The liver pathology observed is likely to be immune-mediated.
- Target Species
- Product Form
- Inactivated Hepatitis A virus - liquid
- Hepatitis A virus, strain HM-175, cultured in FRhK 4 , an established cell line from fetal Rhesus monkey kidney. Cells infected with Hepatitis A virus are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and disrupted to release viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with freon. The antigen preparation is inactivated by incubating with formaldehyde for 96 hours.
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
- None present
- 91% of internal reference standard
- Approx. Protein Concentrations
- Total protein concentration 0.26 mg/ml
- Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
- 18 months from date of despatch
- For research purposes only
Applications of Hepatitis A Virus
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- Instructions For Use
- This product should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.