Nerve Growth Factor 2.5S

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Native Mouse Nerve Growth Factor 2.5S

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
6620-1004 E 10 µg
Nerve growth factor (NGF) is a protein secreted by the target of a neuron that stimulates the growth and differentiation of the sympathetic and certain sensory nerves. Nerve growth factor initially as a 7S proNGF peptide of 3 subunits, alpha, beta (2.5S) and gamma, with the beta (2.5S) subunit having sole responsibility for nerve and growth stimulating activity.

Product Details

Target Species
Mouse
Product Form
Purified NGF from mouse submaxillaries - lyophilised
Reconstitution
Dissolve product in a small volume of sterile distilled water. Stability may be enhanced by the addition of albumin.
Preparation
Prepared by a modification of the method by Bocchini and Angeletti (1). Bioassayed in a rat pheochromocytoma cell line (2).
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
None present
Purity
SDS PAGE: >95%

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P01139 Related reagents
Entrez Gene
Ngf Related reagents
GO Terms
GO:0005057 receptor signaling protein activity
GO:0005788 endoplasmic reticulum lumen
GO:0007422 peripheral nervous system development
GO:0008083 growth factor activity
GO:0019233 sensory perception of pain
GO:0043524 negative regulation of neuron apoptosis
GO:0045773 positive regulation of axon extension
GO:0046928 regulation of neurotransmitter secretion
Regulatory
For research purposes only

Applications of Nerve Growth Factor 2.5S

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

References for Nerve Growth Factor 2.5S

  1. Bocchini, V. & Angeletti, P.U. (1969) The nerve growth factor: purification as a 30,000-molecular-weight protein.
    Proc Natl Acad Sci U S A. 64 (2): 787-94.
  2. Green, L.A. (1977) A quantitative bioassay for nerve growth factor (NGF) activity employing a clonal pheochromocytoma cell line.
    Brain Res. 133 (2): 350-3.
  3. Sun, H.L. & Jiang, T. (2015) The structure of nerve growth factor in complex with lysophosphatidylinositol.
    Acta Crystallogr F Struct Biol Commun. 71 (Pt 7): 906-12.