Nerve growth factor (NGF) is a protein secreted by the target of a neuron that stimulates the growth and differentiation of the sympathetic and certain sensory nerves. Nerve growth factor initially as a 7S proNGF peptide of 3 subunits, alpha, beta (2.5S) and gamma, with the beta (2.5S) subunit having sole responsibility for nerve and growth stimulating activity.
- Target Species
- Product Form
- Purified NGF from mouse submaxillaries - lyophilised
- Dissolve product in a small volume of sterile distilled water. Stability may be enhanced by the addition of albumin.
- Prepared by a modification of the method by Bocchini and Angeletti (1). Bioassayed in a rat pheochromocytoma cell line (2).
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- None present
- SDS PAGE: >95%
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
- 12 months from date of despatch.
- Entrez Gene
- GO Terms
receptor signaling protein activity
endoplasmic reticulum lumen
peripheral nervous system development
growth factor activity
sensory perception of pain
negative regulation of neuron apoptosis
positive regulation of axon extension
regulation of neurotransmitter secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Nerve Growth Factor 2.5S
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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