C1q

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Native Human C1q

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2221-5504 E 1 mg
Native Human Complement Component C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis.

Product Details

Target Species
Human
Product Form
Purified C1q from human serum - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
The solution may appear cloudy following reconstitution
Buffer Solution
0.01M Na2EDTA
0.3M NaCl, pH 7.5
Preservative Stabilisers
None present
Purity
SDS PAGE: >96%
Single band at 410kDa in PAGE.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml after reconstitution

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of reconstitution.

More Information

UniProt
P02745 Related reagents
P02747 Related reagents
P02746 Related reagents
Entrez Gene
C1QA Related reagents
C1QB Related reagents
C1QC Related reagents
GO Terms
GO:0005602 complement component C1 complex
GO:0006958 complement activation, classical pathway
GO:0007267 cell-cell signaling
GO:0045087 innate immune response
GO:0005576 extracellular region
GO:0030853 negative regulation of granulocyte differentiation
GO:0045650 negative regulation of macrophage differentiation
Regulatory
For research purposes only

Applications of C1q

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Application Based External Images

ELISA

Product Specific References

References for C1q

  1. Wenderfer, S.E. et al. (2007) Analysis of C4 and the C4 binding protein in the MRL/lpr mouse.
    Arthritis Res Ther. 9: R114.
  2. Kanda, Y. et al. (2007) Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types.
    Glycobiology. 17 (1): 104-18.
  3. Kao, D. et al. (2015) A Monosaccharide Residue Is Sufficient to Maintain Mouse and Human IgG Subclass Activity and Directs IgG Effector Functions to Cellular Fc Receptors
    Cell Reports. 6 Dec [Epub ahead of print]