Protein G

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Recombinant Protein G

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7860-0104 E 1 mg
Binds Fc-fragment of all subclasses of IgG from human, mouse, rat, rabbit, sheep and goat. Does not bind human IgM, IgA, IgD or albumin.

Product Details

Target Species
Bacterial
Product Form
Recombinant protein - lyophilised
Reconstitution
Reconstitute with 1.0 ml sterile distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in E. coli and purified by affinity chromatography and ion exchange chromatography.
Preservative Stabilisers
None present.
Purity
SDS PAGE: >95%
Molecular Weight
MW: 26 kD

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Protein G

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.