Protein G
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Binds Fc-fragment of all subclasses of IgG from human, mouse, rat, rabbit, sheep and goat. Does not bind human IgM, IgA, IgD or albumin.
Product Details
- Target Species
- Bacterial
- Product Form
- Recombinant protein - lyophilised
- Reconstitution
- Reconstitute with 1.0 ml sterile distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in E. coli and purified by affinity chromatography and ion exchange chromatography.
- Preservative Stabilisers
- None present.
- Purity
- SDS PAGE: >95%
- Molecular Weight
- MW: 26 kD
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Protein G
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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