Chlamydia trachomatis

100% Secure

Chlamydia trachomatis

Product Type
Antigen
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MPP014 E 1 ml
Chlamydia trachomatis is a non-motile, Gram-negative human pathogen. It undergoes a unique biphasic developmental cycle, forming elementary bodies extracellularly and distinctive intracellular inclusions. The elementary body is infectious but metabolically inactive, cannot replicate and lacks detectable peptidoglycan.

Chlamydia trachomatis is a common sexually transmitted bacterium. Chlamydia infection may cause proctitis, urethritis, trachoma (an eye infection and the leading cause of blindness worldwide) and infertility in both sexes. Prostatitis and epididymitis may occur in men and in women infection might lead to acute or chronic pelvic pain, pelvic inflammatory disease, cervicitis and ectopic pregnancy. In neonates the pathogen may cause trachoma and pulmonary complications.

Chlamydia trachomatis (MPP014) is a heat inactivated saline lysate preparation of Chlamydia trachomatis. This preparation is useful for applications that require low concentrations of contaminating protein.

Product Details

Target Species
Bacterial
Product Form
Inactivated Chlamydia trachomatis, LGV type II strain 434, saline lysate - frozen
Preparation
Optimally infected cells are washed and disrupted into a small volume of phosphate buffered saline, subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris.

The preparation is inactivated by heat treatment and 0.05% Triton X-100. Inactivation is verified by two blind passages on permissive cells showing no inclusion bodies.
Buffer Solution
Phosphate buffered saline
0.05% Triton X-100
Preservative Stabilisers
None present
Activity
This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately.
Approx. Protein Concentrations
Total protein concentration 4.2mg/ml.

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Chlamydia trachomatis

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.