Antigen Retrieval Buffer, pH4.8

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  • Antigen Retrieval Buffer, pH4.8
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF027APdatasheet pdfdatasheet pdf500 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Intended Use
      Bio-Rad's Antigen unmasking fluid is a heat induced, epitope retrieval system. The acidic formulation is presented at pH4.8 and is suitable for use with antibodies that have not worked well with the universal formulation. Basic and universal formulations are also available from Bio-Rad - see "recommended useful reagents" at the bottom of the datasheet for further information. The universal formulation is suitable for use with most antibodies and gives excellent preservation of tissue morphology. Like the acidic form, the basic form, may be of value for use with antibodies that do not work well with the universal formulation. These buffers may dramatically increase staining intensity but may also have an effect upon tissue morphology.
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Ready to use
    • Storage
      Store at room temperature. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Deparaffinize sections and re-hydrate

      2. Pre-heat retrieval buffer to 92oC *.

      3. Place slides in buffer and incubate 10 minutes at 92oC

      *.4. Allow to cool for 5-10 minutes.

      5. Rinse in distilled water or PBS, proceed with staining.

      * N.B. Optimal unmasking is a function of time and temperature. Temperature (range 90oC-100oC) and time (5-30 minutes) should be determined by each investigator.

    Additional Antigen Retrieval Buffer, pH4.8 Formats

    Formats Applications Sizes available
    Antigen Retrieval Buffer, pH4.8 : Ready To Use P 500 ml
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH4.8BUF027C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          Antigen Retrieval Buffer, pH10.0BUF026A500 mlP

          Recommended Positive Controls

            Histology Controls

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