Annexin V Kit

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  • Annexin V:APC Assay Kit
  • Annexin V:Pe Assay Kit
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Kits
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ANNEX200APCFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf200 Tests
    ANNEX200APC
    ANNEX200PEFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf200 Tests
    ANNEX200PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This test employs the property of Annexin V to bind to the membrane phospholipid phosphatidylserine (PS) in the presence of Ca2+. PS is exposed at the cell surface during the early stages of apoptosis. Detection of PS is a very sensitive method for detecting cells entering apoptosis, at a time point considerably ahead of nuclear changes such as DNA degradation.

      The conjugation protocol used to prepare this product has not changed the native phospholipid binding properties of Annexin V. This protocol is designed to measure apoptosis easily and quickly in a sample of suspended cells.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.02%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Annexin V:APC200 tests
      Propidium Iodide Staining Solution2X 100 tests
      10X Binding Buffer100 ml
      Annexin V:PE200 tests
      7-AAD Viability Staining Solution2X 100 tests
      10X Binding Buffer100 ml
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch
    • GO Terms
      anti-apoptosis
      calcium-dependent phospholipid binding
      phospholipase inhibitor activity
      calcium ion binding
      cytoplasm
      signal transduction
      blood coagulation
      negative regulation of coagulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      1) Dilute the10X binding buffer to 1X in distilled water (1 ml binding buffer + 9 ml distilled water).

      2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X binding buffer.

      3) Resuspend cells in 1X binding buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

      4) Add 5 μl of APC conjugated Annexin V to 100 μl of the cell suspension.

      5) Mix and incubate for 10 to15 minutes at room temperature.

      6) Wash cells in 1X binding buffer.

      7) Resuspend cells in 200 μl of 1X binding buffer.

      8) Add 5 μl of Propidium Iodide Staining Solution.

      9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.
    • Instructions For Use
      1) Dilute the10X Binding Buffer to 1X in distilled water (1 ml Binding Buffer + 9 ml distilled water).

      2) Wash cells once in PBS by gentle shaking or pipetting up and down. Then wash once in 1X Binding Buffer.

      3) Resuspend cells in 1X Binding Buffer, adjusting to a cell density of 1-5 x 106 cells/ml.

      4) Add 5 μl of PE conjugated Annexin V to 100 μl of the cell suspension.

      5) Mix and incubate for 10 to15 minutes at room temperature.

      6) Wash cells in 1X Binding Buffer.

      7) Resuspend cells in 200 μl of 1X Binding Buffer.

      8) Add 5 μl of 7-AAD Viability Staining Solution and incubate for 5 minutes at room temperature.

      9) Analyse by flow cytometry within 4 hours. Store at 2-8°C in the dark.

    Additional Annexin V Kit Formats

    Formats Applications Sizes available
    Annexin V Kit : APC F 200 Tests
    Annexin V Kit : RPE F 200 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Annexin V:APC Assay KitANNEX50APC50 TestsF
          ANNEX50APC
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX20B20 TestsF
          ANNEX20B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Annexin V:FITC Assay KitANNEX20F20 TestsF
          ANNEX20F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F
          Annexin V:PE Assay KitANNEX200PE200 TestsF
          ANNEX200PE
          Annexin V:PE Assay KitANNEX50PE50 TestsF
          ANNEX50PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Annexin V:APC Assay KitANNEX200APC200 TestsF
          ANNEX200APC
          Annexin V:APC Assay KitANNEX50APC50 TestsF
          ANNEX50APC
          Annexin V:Biotin Assay KitANNEX100B100 TestsF
          ANNEX100B
          Annexin V:Biotin Assay KitANNEX20B20 TestsF
          ANNEX20B
          Annexin V:Biotin Assay KitANNEX300B300 TestsF
          ANNEX300B
          Annexin V:FITC Assay KitANNEX100F100 TestsF
          ANNEX100F
          Annexin V:FITC Assay KitANNEX20F20 TestsF
          ANNEX20F
          Annexin V:FITC Assay KitANNEX300F300 TestsF
          ANNEX300F
          Annexin V:PE Assay KitANNEX50PE50 TestsF
          ANNEX50PE

          Recommended Positive Controls

            Histology Controls

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