Alemtuzumab Antibody | AbD16942_hIgG1

//www.bio-rad-antibodies.com/alemtuzumab-antibody-abd16942-higg1-hca175.html?RenderPageSpecific=1
Alemtuzumab Antibody | AbD16942_hIgG1 gallery image 1

Alemtuzumab bridging ELISA using Human anti Alemtuzumab (HCA174) as the coating antibody; Alemtuzumab as the antigen and Human anti Alemtuzumab:HRP (HCA175P) as the detection antibody

Enlarge
Alemtuzumab Antibody | AbD16942_hIgG1 gallery image 2

Anti-drug-antibody (ADA) assay, bridging format: Alemtuzumab was used as the coating reagent followed by human anti-Alemtuzumab (HCA175). Detection was performed by HRP conjugated Alemtuzumab

Enlarge
Alemtuzumab Antibody | AbD16942_hIgG1 gallery image 3

HCA175 specificity ELISA using various antigens (A: Human serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Bevacizumab, E: Ustekinumab, F: Infliximab, G: Adalimumab, H: Alemtuzumab) and Human anti Alemtuzumab:HRP (HCA175P) as detection reagent

Enlarge
Alemtuzumab Antibody | AbD16942_hIgG1 gallery image 4

Binding of FITC conjugated Alemtuzumab to human lymphocytes (blue unshaded histogram) and subsequent inhibition of binding (red shaded histogram) in the presence of a 5 fold molar excess of HCA175 (A) or the corresponding negative control (B)

Enlarge
  • Alemtuzumab Antibody | AbD16942_hIgG1 thumbnail image 1
  • Alemtuzumab Antibody | AbD16942_hIgG1 thumbnail image 2
  • Alemtuzumab Antibody | AbD16942_hIgG1 thumbnail image 3
  • Alemtuzumab Antibody | AbD16942_hIgG1 thumbnail image 4
  • Human anti Alemtuzumab:HRP
  • Human anti Alemtuzumab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format; it is suitable for bioanalytical method development for alemtuzumab or biosimilars. It is recommended as a the detection antibody in a PK bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD16942_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA175PEdatasheet pdfdatasheet pdf0.1 mg
      HCA175P
      HCA175Edatasheet pdfdatasheet pdf0.1 mg
      HCA175
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti Alemtuzumab, clone AbD16942_hIgG1, is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody.

        Clone AbD16942_hIgG1 can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. The HRP conjugated format of this antibody (HCA175P) may be used in a bridging ELISA with Human anti-alemtuzumab antibody, clone AbD16728.

        Alemtuzumab is a drug used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

        Clone AbD16942_hIgG1 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of Rat anti Human CD52 antibody, clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

        View a summary of all anti-alemtuzumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A.
        Purified IgG prepared by affinity chromatography on Protein A.
      • Preservative Stabilisers
        0.01% Thiomersal
        0.09% Sodium Azide (NaN3).
      • Immunogen
        Alemtuzumab.
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=2.4 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
      • Approx. Protein Concentrations
        Total protein concentration 0.1 mg/ml.
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
        Phosphate buffered saline.
      • Storage
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA174 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in an indirect ELISA or as a detection antibody when conjugated to HRP in a bridging ELISA together with HCA174 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay.
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Alemtuzumab Antibody Formats

      Formats Clone Applications Sizes available
      Alemtuzumab Antibody : Purified AbD16942_hIgG1 E 0.1 mg
      Alemtuzumab Antibody : HRP AbD16942_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti AlemtuzumabHCA1740.1 mgE
            HCA174
            Rat anti Human CD52MCA16420.2 mgC, CT, E, F, IP, P, RE, WB
            MCA1642
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti AlemtuzumabHCA1740.1 mgE
            HCA174
            Rat anti Human CD52MCA16420.2 mgC, CT, E, F, IP, P, RE, WB
            MCA1642
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

                Write your review

                You may also be interested in...