Alcohol Dehydrogenase

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  • Native Yeast Alcohol Dehydrogenase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0240-0504E, FNdatasheet pdfdatasheet pdf75000 Units
    0240-0504
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
      Semi-purified ADH from yeast - lyophilised
    • Reconstitution
      Soluble in distilled water and dilute buffers.
    • Preparation
    • Preservative Stabilisers
      None present
    • Activity
      One unit of enzyme catalyzes the reduction of 1 µmole NAD+ per minute at 25ºC and pH 8.8.

      The activity of 0240-0504 is determined using a procedure as published by Vallee, B.L. and Hoch, F.L., Proc. Nat. Acad. Sci. USA, 41: 327-338 1955.
    • Purity
      Approximately 80% protein by Lowry
    • Approx. Protein Concentrations
    • Molecular Weight
      141 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      plasma membrane enriched fraction
      protein binding
      NADH oxidation
      methylglyoxal reductase (NADH-dependent) activity
      alcohol dehydrogenase (NAD) activity
      cytoplasm
      zinc ion binding
      ethanol biosynthetic process involved in glucose fermentation to ethanol
      amino acid catabolic process to alcohol via Ehrlich pathway
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
      Widely used for the determination of ethanol and as part of coupled-enzymic systems for the determination of metabolites in biological fluids.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Reagents:
      0.05 M Sodium pyrophosphate buffer, pH 8.8.
      96% Ethanol (substrate).
      0.025 M NAD+ (16.7 mg/ml) in buffer. Prepare fresh.
      0.01 M Sodium phosphate buffer, pH 7.5, containing 0.1% bovine serum albumin (BSA).
      Alcohol dehydrogenase (enzyme) - Dissolve sufficient amount of enzyme in 0.01 M sodium phosphate buffer containing 0.1% BSA, pH 7.5, to give a concentration of 0.1-0.5 U/ml. Prepare fresh immediately prior to assay.

      Procedure:
      1. Set spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.
      2. In a cuvette pipette the following reagents in the amounts indicated:
      Sodium pyrophosphate buffer 1.4 ml
      NAD+ 1.4 ml
      Ethanol (substrate) 0.1 ml

      3. Incubate cuvette in spectrophotometer, at 25°C for 5 min. to achieve temperature equilibration and then record absorbance at 340 nm (blank).
      4. Initiate the reaction by adding 0.1 ml of ADH (enzyme) solution to the cuvette. Record the increase in absorbance at 340 nm for 5 min.
      5. Calculate the E340nm/min

    Additional Alcohol Dehydrogenase Formats

    Formats Applications Sizes available
    Alcohol Dehydrogenase : Purified E, FN 75000 Units
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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