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AEC Buffer

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  • AEC Substrate Buffer
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF020C, Pdatasheet pdfdatasheet pdf2 X 50ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Intended Use
      Bio-Rad's AEC substrate buffer is intended for use with AEC chromogen BUF019B for use in immunohistochemistry procedures. It produces a red stain at the site of antigen detected by immunohistochemical staining utilising horseradish peroxidase enzyme labels.
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC.


      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Dilute AEC Chromogen concentrate BUF019B 1:50 with AEC Substrate Buffer.

      2. Apply mixture to tissue section.

      3. Incubate the slides for 5 - 10 min at room temperature.

      4. Rinse tissue sections with deionized water.

      5. Counterstain and mount using aqueous mounting media.

      6. AEC induces a red precipitate with almost no background.

    Additional AEC Buffer Formats

    Formats Applications Sizes available
    AEC Buffer : Reagent C, P 2 X 50ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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