Adalimumab Antibody | AbD18754

Adalimumab Antibody | AbD18754 gallery image 1

HCA206 specificity ELISA using various antigens as coating reagents. Human anti-Adalimumab antibody (HCA206) was used as the primary antibody followed by an anti-His tag:HRP as a detection reagent

Adalimumab Antibody | AbD18754 gallery image 2

HCA206 Antigen-Capture Assay using the Adalimumab/TNFα complex binder HCA206 for detection. Human TNFα was used as the coating reagent followed by Adalimumab spiked into 10% human serum. Human anti-Adalimumab/TNFα antibody HCA206 (conjugated to HRP) was used as the detection reagent

Adalimumab Antibody | AbD18754 gallery image 3

HCA206 specificity titration ELISA. Various antigens (black: TNFα/Adalimumab; red: TNFα/Infliximab; green: TNFα; dark blue: Adalimumab; light blue: Infliximab; pink: human IgG kappa and yellow: BSA) were used as coating reagents. For the antigen complexes, either Adalimumab or Infliximab was then added. Detection was performed using HRP-conjugated HCA206 in a dilution series

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  • Human anti Adalimumab (Drug/Target Complex)
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    Anti-adalimumab drug-target complex antibody specifically recognizes the adalimumab-TNF alpha complex exclusively and detects adalimumab or biosimilars only when bound to TNF alpha. This antibody is in monovalent Fab format and is suitable for a PK antigen capture format assay.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA206Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti Adalimumab, clone AbD18754, is an antibody that specifically recognizes the adalimumab monoclonal antibody while complexed with TNFα. As clone AbD18754 is a complex-specific antibody that recognizes adalimumab bound to its target, TNFα, it does not recognize either free adalimumab or unbound TNFα.

        Clone AbD18754 can be used in assays to measure adalimumab that has been captured via immobilised TNFα. It can also be used to measure adalimumab/TNFα and biosimilar products using bioanalytical assays.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        Veiw a summary of all anti-adalimumab antibodies
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=67 nM by real time, label-free molecular interaction analysis on immobilized adalimumab/TNFα complex.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira® is a registered trademark of Abbott Laboratories.
        His-tag is a registered trademark of EMD Biosciences.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA on Adalimumab/TNFα complexes or as detection antibody, when conjugated to HRP, for Adalimumab bound to TNFα.
        Protocol: PK antigen capture ELISA to measure bound drug exclusively
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18754 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            Hispec Assay DiluentBUF049A50 mlIY
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB

            Recommended Positive Controls

              Histology Controls

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