Adalimumab Antibody | AbD18655_hIgG1

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Adalimumab Antibody | AbD18655_hIgG1 gallery image 1

HCA271 specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti-Adalimumab antibody (HCA204) was used as the detection reagent

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HCA204 PK assay - bridging format. Human anti-Adalimumab (HCA202) was used as the coating antibody followed by Adalimumab spiked into 10% human serum as the antigen. Detection was performed by adding HRP conjugated Human anti-Adalimumab antibody HCA204

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HCA204 Anti-Drug-Antibody (ADA) assay, bridging format. Adalimumab was used as the coating reagent followed by anti-Adalimumab antibodies in human IgG1 format (black squares HCA203; red circles HCA204; green triangles HCA205). Detection was performed with HRP conjugated Adalimumab

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Inhibition of Adalimumab binding to TNFα by HCA204. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA204) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using HRP conjugated HCA207 (Human anti Adalimumab-TNFα complex specific) antibody

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  • Human anti Adalimumab
  • Human anti Adalimumab:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-adalimumab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in fully human IgG1 format; it is ideal for bioanalytical assay development to measure levels of adalimumab or biosimilars. It is recommended as a detection antibody in a PK bridging ELISA with HCA202.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18655_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA204Edatasheet pdfdatasheet pdf0.1 mg
      HCA204
      HCA204PEdatasheet pdfdatasheet pdf0.1 mg
      HCA204P
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti Adalimumab, clone AbD18655_hIgG1, is an ultra-high affinity anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. Clone AbD18655_hIgG1 can be used to measure the levels of adalimumab and biosimilar products using bioanalytical assays. It may also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient serum.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        View a summary of all anti-adalimumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Adalimumab (Humira®)
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.06 nM by real time, label-free molecular interaction analysis on immobilized adalimumab
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
        Total protein concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
      • Shelf Life
        12 months from date of despatch.
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira is a registered trademark of Abbott Laboratories.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira® is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or, when conjugated to HRP, in a bridging ELISA together with HCA202 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay.
        Protocol: ADA bridging ELISA
      • ELISA
        This product may be used as a detection reagent in a sandwich ELISA together with HCA202 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18655_hIgG1 E 0.1 mg
      Adalimumab Antibody : HRP AbD18655_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            MCA647P
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti AdalimumabHCA2020.1 mgE
            HCA202
            Human anti AdalimumabHCA2030.1 mgE
            HCA203
            Human anti AdalimumabHCA2050.1 mgE
            HCA205
            Human anti Adalimumab (Drug/Target Complex)HCA2070.1 mgE
            HCA207
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti AdalimumabHCA2020.1 mgE
            HCA202
            Human anti AdalimumabHCA2030.1 mgE
            HCA203
            Human anti AdalimumabHCA2050.1 mgE
            HCA205
            Human anti Adalimumab (Drug/Target Complex)HCA2070.1 mgE
            HCA207
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB
            PHP051

            Recommended Positive Controls

              Histology Controls

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