Adalimumab Antibody | AbD18655_hIgE

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Human anti adalimumab specificity ELISA
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti adalimumab (clone AbD18655_hIgE) at a concentration of 2 μg/ml followed by Mouse anti human IgE conjugated to HRP (0100-0413P) in HISPEC diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate.

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ELISA using anti adalimumab antibody with IgE isotype for ADA assay development
A microtiter plate was coated overnight with adalimumab, human IgG1/kappa or human IgG1/lambda control at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti Adalimumab (clone AbD18655_hIgE) titrated to the given concentrations in PBST+10% NHS, followed by Mouse anti human IgE conjugated to HRP (0100-0413P) in HISPEC diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is presented as the mean of three measurements.

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  • Human anti Adalimumab
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    Anti-adalimumab antibody is a recombinant, inhibitory, high affinity anti-idiotypic antibody in fully human IgE format. It is recommended as a reference standard in ADA assays to measure levels of patient anti-drug antibodies with IgE isotype.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18655_hIgE
    • Isotype
      IgE
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA271Edatasheet pdfdatasheet pdf0.1 mg
      HCA271
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti Adalimumab, clone AbD18655_hIgE, is an ultra-high affinity, recombinant anti-idiotypic antibody that specifically recognizes adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. This antibody is derived from the same parent clone as HCA204 and has been converted to an IgE isotype.

        Repeated administration of biotherapeutic drugs can result in the induction of anti-drug antibodies (ADAs), which may affect efficacy of the drug and may cause adverse events including hypersensitivity reactions. Most ADAs have isotype IgG, but ADAs with isotype IgE can potentially elicit anaphylactic reactions. The FDA draft guidance for immunogenicity testing of therapeutic proteins indicates that where the risk of anaphylaxis is a concern, assays should be developed to investigate ADAs with IgE isotype.

        Clone AbD18655_hIgE is a fully human antibody with isotype IgE, ideal for use as a reference standard in an ELISA to measure IgE ADAs in patient serum.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        View a summary of all anti-adalimumab antibodies.
      • Intended Use
      • Product Form
        Human IgE antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgE prepared by affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Adalimumab (Humira®)
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.06 nM by real time, label-free molecular interaction analysis on immobilized adalimumab
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA.
        This antibody is fully human and can be used as a reference standard in an ADA assay.
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18655_hIgE E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Human IgE:HRP0100-0413P0.1 mgE
        0100-0413P
        Mouse anti Human IgE:HRPMCA2116P0.5 mgE
        MCA2116P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Hispec Assay DiluentBUF049B125 mlIY
            BUF049B

            Recommended Positive Controls

              Histology Controls

                Further Reading

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