Adalimumab Antibody | AbD18654_hIgG1

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HCA203 specificity ELISA using various antigens as coating reagents. HRP conjugated Human anti-Adalimumab antibody (HCA203) was used as the detection reagent

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HCA203 Anti-Drug-Antibody (ADA) assay, bridging format. Adalimumab was used as the coating reagent followed by anti-Adalimumab antibodies in human IgG1 format (black squares HCA203; red circles HCA204; green triangles HCA205). Detection was performed with HRP conjugated Adalimumab

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Inhibition of Adalimumab binding to TNFα by HCA203. TNFα (PHP051) was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA203) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using HRP conjugated HCA207 (Human anti Adalimumab-TNFα complex specific) antibody

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  • Human anti Adalimumab
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    Anti-adalimumab antibody is a recombinant, neutralizing anti-idiotypic antibody in fully human IgG1 format, suitable for bioanalytical method development for adalimumab and biosimilars. It can be used as the detection antibody in ELISA and as a reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA203Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti Adalimumab, clone AbD18654_hIgG1, is a high affinity anti-idiotypic antibody that specifically recognizes the adalimumab monoclonal antibody and inhibits the binding of adalimumab to its target, TNFα. Clone AbD18654_hIgG1 can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays. It may also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient serum.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        View a summary of all anti adalimumab antibodies.
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.15 nM by real time, label-free molecular interaction analysis on immobilized Adalimumab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira® is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or, when conjugated to HRP, as a detection reagent in a bridging ELISA together with HCA202 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
        This antibody is fully human and can be used as a reference standard in an ADA assay.
        Protocol: ADA bridging ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18654_hIgG1 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            Hispec Assay DiluentBUF049A50 mlIY
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB

            Recommended Positive Controls

              Histology Controls

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