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Mouse anti 5′-Methylcytidine antibody, clone 33D3 used for the demonstration of 5′-methylcytosine in mouse embryonic tissue by immunohistofluorescence.
Changes in 5meC and 5hmC staining in each lineage formed during implantation in vitro. (A) vi, vii, viii, ix, x, xi, xii, xiii, xiv) staining of outgrowths for 5meC (green), 5hmC (red), and DNA (DAPI, blue) of a vi) z-stack projection in the x–y dimension and vii) cross-section in x–z dimension; viii, ix, x) single confocal sections at progressive heights along the z-axis through the embryo shown in vi. xi, xi, xii, xiii) Higher power images of individual xi) TGC, xii) small TB, and xiii) hypoblast cells respectively.
From: Li Y, Seah MK, O'Neill C.
Mapping global changes in nuclear cytosine base modifications in the early mouse embryo.
Reproduction. 2016 Feb;151(2):83-95.